Listeria Fermentation Broth

[所属分类:培养基配方] [发布时间:2021-5-7] [发布人:网站管理员2] [阅读次数:] [返回]

Listeria Fermentation Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Proteose peptone No. 3...............................................................10.0g
NaCl..............................................................................................5.0g
Beef extract...................................................................................1.0g
Bromcresol Purple........................................................................0.1g
Carbohydrate solution..............................................................10.0mL
Carbohydrate Solution:
Composition per 10.0mL:
Carbohydrate.................................................................................5.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize. Use glucose, salicin, rhamnose, dulcitol, or raffinose.
Preparation of Medium: Add components, except carbohydrate
solution, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile
carbohydrate solution. Mix thoroughly. Aseptically distribute into ster-
ile tubes or flasks.
Use: For the cultivation and differentiation of Listeria species based
on the fermentation of glucose, salicin, rhamnose, dulcitol, and
raffinose.