Listeria Enrichment Broth, FDA (LEB, FDA)

[所属分类:培养基配方] [发布时间:2021-5-6] [发布人:网站管理员2] [阅读次数:] [返回]

Listeria Enrichment Broth, FDA
(LEB, FDA)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Soybean casein digest broth yeast extract.....................................1.0L
Nalidixic acid solution...............................................................8.0mL
Cycloheximide solution.............................................................5.1mL
Acriflavin·HCl solution .............................................................3.0mL
pH 7.3 ± 0.2 at 25°C
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-

mation and inhalation.

Soybean Casein Digest Broth Yeast Extract:
Composition per liter:
Pancreatic digest of casein..........................................................17.0g
Yeast extract..................................................................................6.0g
NaCl..............................................................................................5.0g
Papaic digest of soybean meal......................................................3.0g
K 2 HPO 4 .........................................................................................2.5g
Glucose.........................................................................................2.5g
Source : This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Soybean Casein Digest Broth Yeast Extract:
Add components to distilled/deionized water and bring volume to
1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.
Nalidixic Acid Solution:
Composition per 100.0mL:
Nalidixic acid................................................................................0.5g
Preparation of Nalidixic Acid Solution: Add nalidixic acid to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Filter sterilize.
Cycloheximide Solution:
Composition per 100.0mL:
Cycloheximide..............................................................................1.5g
Ethanol.....................................................................................40.0mL
Preparation of Cycloheximide Solution: Add cycloheximide to
40.0mL of ethanol. Mix thoroughly. Bring volume to 100.0mL with
distilled/deionized water. Filter sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Acriflavin·HCl Solution:
Composition per 100.0mL:
Acriflavin·HCl solution ................................................................0.5g
Preparation of Acriflavin·HCl Solution: Add acriflavin·HCl so-
lution to distilled/deionized water and bring volume to 100.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components—except nalidixic acid
solution, acriflavin solution, and cycloheximide solution—to distilled/
deionized water and bring volume to 990.0mL. Mix thoroughly. Gently
heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C. Aseptically add 8.0mL of sterile nalidixic
acid solution, 5.1mL of sterile cycloheximide solution, and 3.0mL of
sterile acriflavin solution. Mix thoroughly. Pour into sterile Petri dishes
or distribute into sterile tubes.
Use: For the isolation and enrichment of Listeria monocytogenes from
foods.