Listeria Enrichment Broth

[所属分类:培养基配方] [发布时间:2021-5-6] [发布人:网站管理员2] [阅读次数:] [返回]

Listeria Enrichment Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein..........................................................17.0g
Yeast extract..................................................................................6.0g
NaCl..............................................................................................5.0g
Papaic digest of soybean meal......................................................3.0g
Glucose .........................................................................................2.5g
K 2 HPO 4 .........................................................................................2.5g
Cycloheximide............................................................................0.05g
Nalidixic acid..............................................................................0.04g
Acriflavine·HCl.........................................................................0.015g
pH 7.3 ± 0.2 at 25°C
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C.
Use: For the isolation and cultivation of Listeria monocytogenes
according to the FDA formula.