Leptotrichia Medium

[所属分类:培养基配方] [发布时间:2021-4-30] [发布人:李佳颐] [阅读次数:] [返回]

Leptotrichia Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein..........................................................10.0g
NaCl..............................................................................................5.0g
Peptone..........................................................................................5.0g
Yeast extract..................................................................................3.0g
Na 2 HPO 4 .......................................................................................2.5g
L- Cysteine·HCl·H 2 O .....................................................................0.5g
Horse serum...........................................................................100.0mL
Tomato decoction.....................................................................50.0mL
pH 7.2–7.4 at 25°C
Tomato Decoction:
Composition per 100.0mL:
Tomatoes..................................................................................50.0mL
Preparation of Tomato Decoction: Mince fresh tomatoes and
measure 50.0mL. Add 50.0mL of tap water. Mix thoroughly. Gently
heat and bring to boiling. Continue boiling for 10 min. Filter through
Whatman #1 filter paper. Autoclave filtrate for 15 min at 15 psi pres-
sure–121°C.
Preparation of Medium: Add components, except horse serum
and tomato decoction, to distilled/deionized water and bring volume to
850.0mL. Mix thoroughly. Gently heat and bring to boiling. Adjust pH
to 7.2–7.4. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
25°C. Aseptically add sterile horse serum and tomato decoction. Mix
thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Leptotrichia buccalis.