Leptotrichia buccalis Medium

[所属分类:培养基配方] [发布时间:2021-4-30] [发布人:李佳颐] [阅读次数:] [返回]

Leptotrichia buccalis Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Nutrient broth................................................................................8.0g
Yeast extract..................................................................................2.0g
Glucose solution ......................................................................10.0mL
L- Cysteine solution...................................................................10.0mL
Hemin solution...........................................................................4.0mL
pH 7.2–7.6 at 25°C
Glucose Solution:
Composition per 10.0mL:
D- Glucose......................................................................................2.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-
ize.
L -Cysteine Solution:
Composition per 10.0mL:
L- Cysteine·HCl·H 2 O......................................................................1.0g
Preparation of  L -Cysteine Solution: Add  L -cysteine·HCl·H 2 O to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Hemin Solution:
Composition per 10.0mL:
Hemin.........................................................................................2.5mg
Triethanolamine (50% solution)..............................................10.0mL
Preparation of Hemin Solution:  Add hemin to 10.0mL of trieth-
anolamine solution. Mix thoroughly.
Preparation of Medium: Add components, except glucose solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile glu-
cose solution. Mix thoroughly. Pour into sterile Petri dishes or distrib-
ute into sterile tubes.
Use:  For the cultivation and maintenance of Leptotrichia buccalis.