Leptospirillum HH Medium (DSMZ Medium 882)

[所属分类:培养基配方] [发布时间:2021-4-30] [发布人:李佳颐] [阅读次数:] [返回]

Leptospirillum HH Medium
(DSMZ Medium 882)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1001.0mL:

Solution A..............................................................................950.0mL
Soultion B................................................................................50.0mL
Trace elements solution .............................................................1.0mL
pH 1.8 ± 0.2 at 25°C
Solution A:
CaCl 2 ·2H 2 O............................................................................147.0mg
(NH 4 ) 2 SO 4 ..............................................................................132.0mg
MgCl 2 ·6H 2 O.............................................................................53.0mg
KH 2 PO 4 ....................................................................................27.0mg
Preparation of Solution A: Add components to distilled/deionized
water and bring volume to 950.0mL. Mix thoroughly. Adjust pH to 1.8
with 10N H 2 SO 4 .  Autoclave for 30 min at 112°C. Cool to room tem-
perature.
Solution B:
FeSO 4 ·7H 2 O................................................................................20.0g
H 2 SO 4 , 0.25N...........................................................................50.0mL
Preparation of Solution B: Add FeSO 4 ·7H 2 O to 50.0mL 0.25N
H 2 SO 4 . Mix thoroughly. The pH should be 1.2. Autoclave for 30 min
at 112°C. Cool to room temperature.
Trace Elements Solution:
ZnCl 2 ........................................................................................68.0mg
CuCl 2 ·2H 2 O .............................................................................67.0mg
CoCl 2 ·6H 2 O .............................................................................64.0mg
MnCl 2 ·2H 2 O.............................................................................62.0mg
H 3 BO 3 ......................................................................................31.0mg
Na 2 MoO 4 ..................................................................................10.0mg
Preparation of Trace Elements Solution:  Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 1.8 with 10N H 2 SO 4 .  Autoclave for 15 min at 15 psi

pressure–121°C. Cool to room temperature.

Preparation of Medium:  Aseptically mix 950.0mL of solution A
and 50.0mL solution B. Mix thoroughly. Aseptically add 1.0mL trace
elements solution. Mix thoroughly. Adjust pH to 1.8.
Use:  For the cultivation of Acidithiobacillus ferrooxidans=Thiobacil-
lus ferrooxidans and Leptospirillum spp.