Leptospirillum ferrooxidans Medium

[所属分类:培养基配方] [发布时间:2021-4-30] [发布人:李佳颐] [阅读次数:] [返回]

Leptospirillum ferrooxidans Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

FeSO 4 ·7H 2 O................................................................................30.0g
CaCl 2 ·2H 2 O...............................................................................0.147g
(NH 4 ) 2 SO 4 ...................................................................................0.13g
KH 2 PO 4 ....................................................................................27.0mg
MgCl 2 ·6H 2 O.............................................................................25.0mg
Trace elements solution .............................................................1.0mL
pH 2.0 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
CoCl 2 ·6H 2 O ................................................................................0.12g
MnCl 2 ·4H 2 O..................................................................................0.1g
Na 2 MoO 4 ·2H 2 O .......................................................................85.2mg
ZnCl 2 ........................................................................................70.0mg
H 3 BO 3 ......................................................................................31.0mg
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add H 2 SO 4 to 900.0mL of distilled/de-
ionized water and bring pH to 3.0. Add components. Mix thoroughly.
Bring volume to 1.0L with distilled/deionized water. Mix thoroughly.
Adjust pH to 2.0 with H 2 SO 4 . Distribute into tubes or flasks. Autoclave
for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Leptospirillum ferrooxidans.