Legume Extract Agar

[所属分类:培养基配方] [发布时间:2021-4-30] [发布人:李佳颐] [阅读次数:] [返回]

Legume Extract Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Alfalfa roots................................................................................35.0g
Agar ............................................................................................20.0g
Soybean meal..............................................................................10.0g
Sucrose........................................................................................10.0g
CaCO 3 ...........................................................................................5.0g
Glucose .........................................................................................5.0g
K 2 HPO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.2g
CaCl 2 .............................................................................................0.1g
NaCl..............................................................................................0.1g
FeCl 3 ..........................................................................................1.0mg
Preparation of Medium: Wash the alfalfa roots well and cut them
up. Add 10.0g of soybean meal. Add three times the volume of dis-
tilled/deionized water. Steam for 1 hr. Let stand at 25°C overnight.
Bring volume to 1.0L with distilled/deionized water. Filter through pa-
per pulp. To the filtrate, add the K 2 HPO 4 , CaCl 2 , MgSO 4 ·7H 2 O, NaCl,
FeCl 3 , and agar. Autoclave for 20 min at 15 psi pressure–121°C. Cool
to 45°–50°C. Add the CaCO 3 , sucrose, and glucose. Mix thoroughly.
Distribute into tubes or flasks. Autoclave for 20 min at 10 psi pressure–
115°C.
Use: For the cultivation of Rhizobium species.