Legionella Selective Agar

[所属分类:培养基配方] [发布时间:2021-4-30] [发布人:李佳颐] [阅读次数:] [返回]

Legionella Selective Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
ACES (2-[(2-amino-2-oxoethyl)-amino]ethane
sulfonic acid) buffer .........................................................10.0g
Yeast extract................................................................................10.0g
Charcoal, activated .......................................................................2.0g
α-Ketoglutarate.............................................................................1.0g
L- Cysteine·HCl·H 2 O solution...................................................10.0mL
Fe 4 (P 2 O 7 ) 3 solution..................................................................10.0mL
Antibiotic solution...................................................................10.0mL
pH 6.85–7.0 at 25°C
Source:  This medium is available as a prepared medium from BD Di-
agnostic Systems.
L -Cysteine·HCl·H 2 O Solution:
Composition per 10.0mL:
L- Cysteine·HCl·H 2 O......................................................................0.4g
Preparation of  L -Cysteine·HCl·H 2 O Solution: Add  L -cyste-
ine·HCl·H 2 O to distilled/deionized water and bring volume to 10.0mL.
Mix thoroughly. Filter sterilize.
Fe 4 (P 2 O 7 ) 3 Solution:
Composition per 10.0mL:
Fe 4 (P 2 O 7 ) 3 ...................................................................................0.25g
Preparation of Fe 4 (P 2 O 7 ) 3 Solution: Add Fe 4 (P 2 O 7 ) 3 to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Antibiotic Solution:
Composition per 10.0mL:
Anisomycin..............................................................................10.0mg
Colistin.....................................................................................3.75mg
Vancomycin ...............................................................................2.0mg
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components—except  L -cyste-
ine·HCl·H 2 O, Fe 4 (P 2 O 7 ) 3 , and antibiotic solutions—to distilled/deionized
water and bring volume to 970.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C. Aseptically add sterile  L -cysteine·HCl·H 2 O, Fe 4 (P 2 O 7 ) 3 , and
antibiotic solutions. Mix thoroughly. Pour into sterile Petri dishes. Swirl
medium while pouring to keep charcoal in suspension.
Use: Legionella selective agar is used in qualitative procedures for the
isolation of Legionella species from clinical and nonclinical speci-
mens.