Lee's Multidifferential HiVeg Agar

[所属分类:培养基配方] [发布时间:2021-4-29] [发布人:李佳颐] [阅读次数:] [返回]

Lee's Multidifferential HiVeg Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Tomato juice, dessicated.............................................................20.0g
Plant hydrolysate No. 3...............................................................20.0g
Agar............................................................................................15.0g
Glucose.......................................................................................10.0g
Yeast extract................................................................................10.0g
CaCO 3 ...........................................................................................5.0g
Calcium pantothenate ...................................................................2.0g
Citric acid......................................................................................1.1g
Polysorbate 80 ..............................................................................0.5g
K 2 HPO 4 .........................................................................................0.5g
KH 2 PO 4 .........................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.2g
FeSO 4 ·7H 2 O................................................................................0.01g
MnSO 4 ·7H 2 O..............................................................................0.01g
NaCl............................................................................................0.01g
Bromcresol Green.....................................................................0.022g
Cycloheximide...........................................................................7.0mg

pH 6.7 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMe-
dia.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Pour into sterile Petri plates while swirling to prevent calcium
carbonate from settling. The medium will have a white precipitate of
calcium carbonate.
Use: For the detection of most organisms commonly encountered in a
brewery. Acid producing bacteria are identified by the development of
a clear zone around the colonies. Further identification is facilitated by
the characteristic color reactions.