Lee's Multidifferential Agar

[所属分类:培养基配方] [发布时间:2021-4-29] [发布人:李佳颐] [阅读次数:] [返回]

Lee's Multidifferential Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Tomato juice, dessicated.............................................................20.0g
Peptonized milk..........................................................................20.0g
Glucose.......................................................................................10.0g
Yeast extract................................................................................10.0g
Agar............................................................................................15.0g
CaCO 3 ...........................................................................................5.0g
Calcium pantothenate ...................................................................2.0g
Citric acid......................................................................................1.1g
Polysorbate 80 ..............................................................................0.5g
K 2 HPO 4 .........................................................................................0.5g
KH 2 PO 4 .........................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.2g
FeSO 4 ·7H 2 O................................................................................0.01g
MnSO 4 ·7H 2 O..............................................................................0.01g
NaCl............................................................................................0.01g
Bromcresol Green.....................................................................0.022g
Cycloheximide...........................................................................7.0mg
pH 5.5 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from HiMe-
dia.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 10 min at 15 psi pressure–121°C. Pour into
sterile Petri plates while swirling to prevent calcium carbonate from
settling. The medium will have a white precipitate of calcium carbon-
ate.
Use: For the detection of most organisms commonly encountered in a
brewery. Acid producing bacteria are identified by the development of
a clear zone around the colonies. Further identification is facilitated by
the characteristic color reactions.