Lecithin Agar

[所属分类:培养基配方] [发布时间:2021-4-29] [发布人:李佳颐] [阅读次数:] [返回]

Lecithin Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Fraction B ..............................................................................500.0mL
Fraction A..............................................................................450.0mL
Fraction C ................................................................................50.0mL
pH 7.2 ± 0.2 at 25°C
Fraction A:
Composition per 500.0mL:
Agar............................................................................................18.0g
Tryptone......................................................................................10.0g
Yeast extract..................................................................................5.0g
Glucose.........................................................................................5.0g
Preparation of Fraction A: Add components to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 43°C.
Fraction B:
Composition per 450.0mL:
Crude soy lecithin.......................................................................30.0g
Preparation of Fraction B: Add crude soy lethicin to distilled/de-
ionized water and bring volume to 450.0mL. Mix thoroughly. Gently
heat and bring to boiling. Swirl to form a viscous sol. Sonicate until ho-
mogeneous. Blending of unheated fraction A in a Waring blender for 2
min at high speed is also satisfactory. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 43°C.

Fraction C:
Composition per 50.0mL:
CaCl 2 .............................................................................................0.6g
Preparation of Fraction C: Add CaCl 2 to distilled/deionized water
and bring volume to 50.0mL. Mix thoroughly. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 43°C.
Preparation of Medium: Combine fractions with gentle swirling.
To prevent separation, immediately pour into sterile Petri plates.
Use: For the detection of microbial phospholipases.