Lead Acetate Agar

[所属分类:培养基配方] [发布时间:2021-4-29] [发布人:李佳颐] [阅读次数:] [返回]

Lead Acetate Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Peptone .......................................................................................15.0g
Proteose peptone...........................................................................5.0g
Glucose.........................................................................................1.0g
Lead acetate..................................................................................0.2g
Na 2 S 2 O 3 ......................................................................................0.08g
pH 6.6 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Allow
tubes to cool in a slanted position.
Use: For the cultivation and differentiation of Gram-negative coliform
bacteria based on H 2 S production. Bacteria that produce H 2 S turn the
medium brown.