LB Medium (LB Broth, Miller) (ATCC Medium 1065) (ATCC Medium 1082)

[所属分类:培养基配方] [发布时间:2021-4-28] [发布人:李佳颐] [阅读次数:] [返回]

LB Medium
(LB Broth, Miller)
(ATCC Medium 1065)
(ATCC Medium 1082)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................10.0g
Pancreatic digest of casein..........................................................10.0g
Yeast extract..................................................................................5.0g
Source:  This medium is available from BD Diagnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation and maintenance of Bacillus subtilis, Coryne-
bacterium glutamicum, Enterobacter cloacae, Erwinia uredovora,
Escherichia coli, Klebsiella oxytoca, and Salmonella choleraesuis.