LB Broth, Modified

[所属分类:培养基配方] [发布时间:2021-4-28] [发布人:李佳颐] [阅读次数:] [返回]

LB Broth, Modified

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein..........................................................10.0g
NaCl..............................................................................................5.8g
Yeast extract..................................................................................5.0g
NaCl solution...........................................................................16.8mL
Glucose solution......................................................................10.0mL
CaCl 2 ·2H 2 O solution..................................................................2.0mL
MgCl 2 solution...........................................................................1.6mL
pH 7.0 ± 0.2 at 25°C
NaCl Solution:
Composition per 100.0mL:
NaCl............................................................................................25.0g
Preparation of NaCl Solution: Add NaCl to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Glucose Solution:
Composition per 100.0mL:
D- Glucose....................................................................................40.0g
Preparation of Glucose Solution: Add  D- glucose to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
CaCl 2 ·2H 2 O Solution:
Composition per 10.0mL:
CaCl 2 ·2H 2 O ..............................................................................0.735g
Preparation of CaCl 2 ·2H 2 O Solution: Add CaCl 2 ·2H 2 O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
MgCl 2 Solution:
Composition per 10.0mL:
MgCl 2 ..........................................................................................0.95g
Preparation of MgCl 2 Solution: Add MgCl 2 to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-

ize.

Preparation of Medium: Add components—except NaCl solution,
glucose solution, CaCl 2 ·2H 2 O solution, and MgCl 2 solution—to dis-
tilled/deionized water and bring volume to 969.6mL. Mix thoroughly.
Adjust pH to 7.0. Autoclave for 30 min at 15 psi pressure–121°C. Cool
to 45°–50°C. Aseptically add 16.8mL of sterile NaCl solution, 10.0mL
of sterile glucose solution, 2.0mL of sterile CaCl 2 ·2H 2 O solution, and
1.6mL of sterile MgCl 2 solution. Mix thoroughly. Aseptically distrib-
ute into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Escherichia coli.