Lactose Medium

[所属分类:培养基配方] [发布时间:2021-4-28] [发布人:李佳颐] [阅读次数:] [返回]

Lactose Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
K 2 HPO 4 .........................................................................................0.5g
(NH 4 ) 2 SO 4 .....................................................................................0.5g
NaCl.........................................................................................50.0mg
Lactose solution.......................................................................50.0mL
pH 7.2 ± 0.2 at 25°C
Lactose Solution:
Composition per 50.0mL:
Lactose..........................................................................................5.0g
Preparation of Lactose Solution:  Add lactose to distilled/deion-
ized water and bring volume to 50.0mL. Mix thoroughly. Autoclave for
15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except lactose solu-
tion, to distilled/deionized water and bring volume to 950.0mL. Mix
thoroughly. Adjust pH to 7.2. Autoclave for 15 min at 15 psi pressure–
121°C. Aseptically add 50.0mL of sterile lactose solution. Mix thor-
oughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of unidentified bacterium ATCC 51468.