Lactose Lecithin Agar

[所属分类:培养基配方] [发布时间:2021-4-28] [发布人:李佳颐] [阅读次数:] [返回]

Lactose Lecithin Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Casein enzymic hydrolysate.....................................................12.65g
Lactose........................................................................................10.0g
Peptic digest of animal tissue .......................................................5.5g
NaCl..............................................................................................5.5g
Yeast extract................................................................................3.85g
Pancreatic digest of heart muscle .................................................3.3g
Corn starch....................................................................................1.1g
Egg lecithin.................................................................................0.66g
L -Cysteine·HCl·H 2 O.....................................................................0.5g
NaN 3 .............................................................................................0.2g
Neomycin sulfate........................................................................0.15g
CaCl 2 ·2H 2 O ................................................................................0.05g
Bromcresol Purple....................................................................0.025g
pH 6.8 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Caution:  Sodium azide has a tendency to form explosive metal azides
with plumbing materials. It is advisable to use enough water to flush
off the disposables.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the isolation and differentiation of histotoxic clostridia from
clinical specimens.