Lactose Casein Hydrolysate Medium

[所属分类:培养基配方] [发布时间:2021-4-28] [发布人:李佳颐] [阅读次数:] [返回]

Lactose Casein Hydrolysate Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Lactose........................................................................................37.5g
Agar............................................................................................15.0g
Casein hydrolysate........................................................................3.0g
KH 2 PO 4 .........................................................................................1.0g
MnSO 4 ..........................................................................................0.5g
Microelements solution .............................................................2.0mL
pH 6.0 ± 0.2 at 25°C
Microelements Solution:
Composition per liter:
Fe(NO 3 ) 3 ·9H 2 O......................................................................723.5mg
ZnSO 4 ·7H 2 O..........................................................................439.8mg
MnSO 4 ·4H 2 O.........................................................................203.0mg
H 2 SO 4 ..................................................................................... variable
Preparation of Microelements Solution:  Add components, one
at a time, to distilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Be sure one component is dissolved before adding the next.
Add sulfuric acid to yield a clear solution.
Preparation of Medium: Add components to distilled/deionized wa-
ter and bring volume to 1.0L. Mix thoroughly. Gently heat until boiling.
Adjust pH to 6.0. Distribute into tubes or flasks. Autoclave for 15 min at
15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Drechslera catenaria.