Lactose Broth

[所属分类:培养基配方] [发布时间:2021-4-28] [发布人:李佳颐] [阅读次数:] [返回]

Lactose Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Lactose..........................................................................................5.0g
Pancreatic digest of gelatin...........................................................5.0g
Beef extract...................................................................................3.0g
pH 6.9 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-

agnostic Systems and Oxoid Unipath.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
containing an inverted Durham tube in 10.0mL volumes. Autoclave for
12 min at 15 psi pressure–121°C. Cool broth quickly to 25°C. For test-
ing water samples with 10.0mL volumes, prepare medium double
strength.
Use: For the detection of lactose-fermenting, Gram-negative coli-
forms, as a preenrichment broth for Salmonella species, and in the
study of lactose fermentation of bacteria in general.