Kunkee Medium

[所属分类:培养基配方] [发布时间:2021-4-25] [发布人:李佳颐] [阅读次数:] [返回]

Kunkee Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1000.88mL:

Pancreatic digest of casein..........................................................20.0g
Glucose .........................................................................................5.0g
Peptone..........................................................................................5.0g
Yeast extract..................................................................................5.0g
Filtered tomato juice..............................................................250.0mL
Ethanol (96% solution)..........................................................176.0mL
Tween™ 80................................................................................0.5mL
pH 5.5 ± 0.2 at 25°C
Preparation of Medium: Add components, except ethanol, to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Dis-
tribute into tubes in 5.0mL volumes. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to room temperature. Aseptically add 0.88mL of
ethanol solution to each tube containing 5.0mL of medium.
Use:  For the cultivation of Lactobacillus fructivorans.