Korthof Medium, Modified

[所属分类:培养基配方] [发布时间:2021-4-25] [发布人:李佳颐] [阅读次数:] [返回]

Korthof Medium, Modified

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..............................................................................................1.4g
Na 2 HPO 4 ·2H 2 O...........................................................................0.88g
Peptone..........................................................................................0.8g
KH 2 PO 4 .......................................................................................0.24g
CaCl 2 ...........................................................................................0.04g
KCl..............................................................................................0.04g
NaHCO 3 ......................................................................................0.02g
Rabbit serum, heat inactivated at 56°C..................................100.0mL
pH 7.2–7.6 at 25°C
Preparation of Medium: Add components, except rabbit serum, to
distilled/deionized water and bring volume to 900.0L. Mix thoroughly.
Gently heat and bring to boiling. Boil for 20 min. Cool overnight at
4°C. Filter through Whatman #2 filter paper. Distribute into tubes or
flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–
56°C. Aseptically add 100.0mL of rabbit serum. Mix thoroughly.
Use: For the cultivation of Leptospira species.