Korthof Medium

[所属分类:培养基配方] [发布时间:2021-4-25] [发布人:李佳颐] [阅读次数:] [返回]

Korthof Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1088.0mL:

NaCl..............................................................................................1.4g
Na 2 HPO 4 ·2H 2 O...........................................................................0.88g
Peptone..........................................................................................0.8g
KH 2 PO 4 .......................................................................................0.24g
CaCl 2 ...........................................................................................0.04g
KCl..............................................................................................0.04g
NaHCO 3 ......................................................................................0.02g
Rabbit serum, inactivated ........................................................80.0mL
Rabbit hemoglobin solution.......................................................8.0mL
pH 7.2 ± 0.2 at 25°C
Rabbit Hemoglobin Solution:
Composition per 20.0mL:
Rabbit blood clot......................................................................10.0mL
Preparation of Rabbit Hemoglobin Solution: Add rabbit blood
clot to 10.0mL of distilled/deionized water. Lyse the clot by freezing
and thawing.
Preparation of Medium: Add components, except rabbit serum
and rabbit hemoglobin solution, to distilled/deionized water and bring
volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Cool
to 25°C. Filter through Whatman #1 filter paper. Distribute into flasks
in 100.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 45°–50°C. Aseptically add 8.0mL of rabbit serum and 0.8mL
of rabbit hemoglobin solution to each flask. Mix thoroughly.
Use: For the cultivation of Leptospira species.