Knisely Medium for Bacillus anthracis

[所属分类:培养基配方] [发布时间:2021-4-25] [发布人:李佳颐] [阅读次数:] [返回]

Knisely Medium for Bacillus anthracis

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Beef heart, solids from infusion................................................500.0g
Agar ............................................................................................15.0g
Pancreatic digest of casein..........................................................10.0g
NaCl..............................................................................................5.0g
EDTA.....................................................................................200.0mg
Lysozyme.................................................................................40.0mg
Thallous acetate .......................................................................40.0mg
Polymyxin..............................................................................30,000U
Preparation of Medium: Add components, except EDTA,
lysozyme, thallous acetate, and polymyxin, to distilled/deionized water
and bring volume to 1.0mL. Mix thoroughly. Gently heat and bring to
boiling. Adjust pH to 7.3. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C. Aseptically add sterile EDTA, lysozyme,
thallous acetate, and polymyxin. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Bacillus anthracis.