KM Agar (Kempler-McKay Agar)

[所属分类:培养基配方] [发布时间:2021-4-25] [发布人:李佳颐] [阅读次数:] [返回]

KM Agar
(Kempler-McKay Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Milk, nonfat ................................................................................10.0g
Glucose.........................................................................................5.0g
Milk protein hydrolysate...............................................................2.5g
Solution 1.................................................................................10.0mL
Solution 2.................................................................................10.0mL
pH 6.6 ± 0.2 at 25°C
Solution 1:
Composition per 100.0mL:
K 3 Fe(CN) 6 ..................................................................................10.0g
Caution: Cyanide is toxic.
Preparation of Solution 1: Add K 3 Fe(CN) 6 to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Solution 2:
Composition per 40.0mL:
Ferric citrate..................................................................................1.0g
Sodium citrate...............................................................................1.0g
Preparation of Solution 2: Add components to distilled/deionized
water and bring volume to 40.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except solution 1 and
solution 2, to distilled/deionized water and bring volume to 980.0mL.
Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 6.6. Au-
toclave for 12 min at 10 psi pressure–115°C. Cool to 45°–50°C. Asepti-
cally add sterile solution 1 and solution 2. Mix thoroughly. Pour into
sterile Petri dishes. Allow plates to dry in the dark at 30°C for 24 hr be-
fore using.
Use: For the isolation and cultivation of acidogenic microorganisms
from foods. For the differentiation of citrate-fermenting lactic bacteria,
such as Lactobacillus lactis subspecies diacetylactis, from the nonci-
trate-fermenting Lactobacillus lactis subspecies cremoris.