Kimmig Fungi HiVeg Agar Base with Kimmig Supplement

[所属分类:培养基配方] [发布时间:2021-4-23] [发布人:李佳颐] [阅读次数:] [返回]

Kimmig Fungi HiVeg Agar Base
with Kimmig Supplement

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose.......................................................................................19.0g
Plant peptone ..............................................................................15.0g
Agar............................................................................................15.0g
NaCl..............................................................................................1.0g
Cycloheximide..............................................................................0.4g
Kimmig selective supplement..................................................10.0mL
Glycerol .....................................................................................5.0mL
pH 6.9 ± 0.2 at 35°C
Source: This medium, without glycerol or Kimmig selective supple-
ment, is available as a premixed powder from HiMedia.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Kimmig Selective Supplement:
Composition per 10.0mL:
Novobiocin ............................................................................200.0mg
Colistin sulfate.........................................................................80.0mg
Preparation of Kimmig Selective Supplement: Add compo-
nents to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add glycerol and then other components,
except Kimmig selective supplement, to distilled/deionized water and
bring volume to 990.0L. Mix thoroughly. Gently heat and bring to boil-
ing. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.
Aseptically add 10.0mL sterile Kimmig selective supplement. Mix
thoroughly Pour into sterile Petri dishes or leave in tubes.
Use: For the assay of fungistatic agents. For agar dilution testing of
antifungal agents. For the cultivation and preservation of various fungi.