Kievskaya Agar (Medium K)

[所属分类:培养基配方] [发布时间:2021-4-23] [发布人:李佳颐] [阅读次数:] [返回]

Kievskaya Agar
(Medium K)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
KNO 3 ............................................................................................1.0g
KH 2 PO 4 ........................................................................................1.0g
K 2 HPO 4 ........................................................................................1.0g
NaCl..............................................................................................1.0g
MgSO 4 ..........................................................................................0.2g
CaCl 2 ..........................................................................................0.02g
FeCl 3 ..........................................................................................1.0mg
pH 6.8–7.0 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 30 min at 3 psi
pressure–105°C. Pour into sterile Petri dishes or leave in tubes. After
inoculation, incubate in an atmosphere of natural gas.
Use: For the cultivation of Rhodococcus luteus, Rhodococcus rhodo-
chrous, Rhodococcus ruber, Rhodococcus species, and other bacteria
that can grow on natural gas.