KG HiVeg Agar Base

[所属分类:培养基配方] [发布时间:2021-4-23] [发布人:李佳颐] [阅读次数:] [返回]

KG HiVeg Agar Base

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................18.0g
Plant peptone.................................................................................1.0g
Yeast extract..................................................................................0.5g
Phenol Red................................................................................0.025g
Egg yolk emulsion, 50%........................................................100.0mL
Polymyxin B solution ................................................................1.0mL
pH 6.8 ± 0.2 at 25°C
Source: This medium, without egg yolk emulsion and polymyxin B
solution, is available as a premixed powder from HiMedia.
Egg Yolk Emulsion, 50%:
Composition per 100.0mL:
Chicken egg yolks............................................................................11
Whole chicken egg.............................................................................1
NaCl (0.9% solution)...............................................................50.0mL
Preparation of Egg Yolk Emulsion, 50%: Soak eggs with 1:100
dilution of saturated mercuric chloride solution for 1 min. Crack eggs
and separate yolks from whites. Mix egg yolks with 1 chicken egg.
Beat to form emulsion. Measure 50.0mL of egg yolk emulsion and add
to 50.0mL of 0.9% NaCl solution. Mix thoroughly. Filter sterilize.
Warm to 45°–50°C.
Polymyxin B Solution:
Composition per 5.0mL:
Polymyxin B sulfate.............................................................500,000U
Preparation of Polymyxin B Solution: Add polymyxin B sulfate
to distilled/deionized water and bring volume to 5.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except egg yolk emul-
sion and polymyxin B solution, to distilled/deionized water and bring
volume to 900.0mL. Mix thoroughly. Adjust pH to 6.8. Autoclave for
15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add
100.0mL of sterile egg yolk emulsion, 50%, and 1.0mL of sterile pol-
ymyxin B solution. Mix thoroughly. Pour into sterile Petri dishes. Al-
low plates to dry in the dark at 30°C for 24 hr before using.
Use: For the cultivation and differentiation of Bacillus cereus.