KF Streptococcus Broth

[所属分类:培养基配方] [发布时间:2021-4-23] [发布人:李佳颐] [阅读次数:] [返回]

KF Streptococcus Broth
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Maltose .......................................................................................20.0g
Sodium glycerophosphate...........................................................10.0g
Yeast extract................................................................................10.0g
NaCl..............................................................................................5.0g
Pancreatic digest of casein............................................................5.0g
Peptic digest of animal tissue .......................................................5.0g
Lactose..........................................................................................1.0g
Na 2 CO 3 .....................................................................................0.636g
NaN 3 .............................................................................................0.4g
Phenol Red................................................................................0.018g
2,3,5-Triphenyltetrazolium chloride solution..........................10.0mL
pH 7.2 ± 0.2 at 25°C
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Source:  This medium is available as a premixed powder from BD Di-

agnostic Systems.

Preparation of Medium:  Add components, except 2,3,5-triphe-
nyltetrazolium chloride solution, to distilled/deionized water and bring
volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add 2,3,5-triphenyltetrazolium chloride solution. Mix thor-
oughly. Aseptically distribute into sterile tubes or flasks.
Use: For the selective cultivation of fecal streptococci.