KC Broth

[所属分类:培养基配方] [发布时间:2021-4-22] [发布人:李佳颐] [阅读次数:] [返回]

KC Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein..........................................................10.0g
KCl................................................................................................5.0g
CaCl 2 solution............................................................................0.5mL
CaCl 2 Solution:
Composition per 10.0mL:
CaCl 2 ·2H 2 O.................................................................................1.47g
Preparation of CaCl 2 Solution: Add CaCl 2 ·2H 2 O to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except CaCl 2 solution,
to distilled/deionized water and bring volume to 999.5mL. Mix thor-
oughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 25°C. Aseptically add 0.5mL of CaCl 2 solu-
tion. Mix thoroughly. Aseptically distribute into sterile tubes.
Use: For the cultivation of Escherichia coli