KC Bottom Agar

[所属分类:培养基配方] [发布时间:2021-4-22] [发布人:李佳颐] [阅读次数:] [返回]

KC Bottom Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................10.0g
Pancreatic digest of casein..........................................................10.0g
KCl................................................................................................2.5g
NaCl..............................................................................................2.5g
CaCl 2 solution............................................................................1.0mL
CaCl 2 Solution:
Composition per 10.0mL:
CaCl 2 ·2H 2 O ................................................................................1.47g
Preparation of CaCl 2 Solution: Add CaCl 2 ·2H 2 O to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-

ilize.

Preparation of Medium: Add components, except CaCl 2 solution,

to distilled/deionized water and bring volume to 999.0mL. Mix thor-
oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add 1.0mL of CaCl 2
solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the cultivation and maintenance of Escherichia coli.