ISP Medium 4 (International Streptomyces Project Medium 4) (Inorganic Salts Starch Agar)

[所属分类:培养基配方] [发布时间:2021-4-21] [发布人:李佳颐] [阅读次数:] [返回]

ISP Medium 4
(International Streptomyces Project Medium 4)
(Inorganic Salts Starch Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................20.0g
Soluble starch..............................................................................10.0g
CaCO 3 ...........................................................................................2.0g
(NH 4 ) 2 SO 4 ....................................................................................2.0g
K 2 HPO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................1.0g
NaCl..............................................................................................1.0g
FeSO 4 ·7H 2 O...............................................................................1.0mg
MnCl 2 ·7H 2 O ..............................................................................1.0mg
ZnSO 4 ·7H 2 O..............................................................................1.0mg
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling with frequent agitation. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes

or leave in tubes.

Use: For characterizing Streptomyces species. For the cultivation and

maintenance of Actinomadura fastidiosa, Actinomadura roseoviolacea,
Actinomadura species, Actinoplanes species, Amycolatopsis mediterra-
nei, Kitasatosporia grisea, Kitasatosporia papulosa, Saccharomono-

spora internatus, Streptomyces species, Streptosporangium species,

Saccharomonospora hirsuta, and Streptoverticillium species.