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ISP Medium 3 (International Streptomyces Project Medium 3) (Oatmeal Agar)
[所属分类:培养基配方] [发布时间:2021-4-21] [发布人:李佳颐] [阅读次数:] [返回]
ISP Medium 3
(International Streptomyces Project Medium 3)
(Oatmeal Agar)
Agar............................................................................................18.0g
Trace salts solution ....................................................................1.0mL
Trace Salts Solution:
Composition per 100.0mL:
FeSO 4 ·7H 2 O..................................................................................0.1g
MnCl 2 ·4H 2 O .................................................................................0.1g
ZnSO 4 ·7H 2 O.................................................................................0.1g
Preparation of Trace Salts Solution: Add components to dis-
tilled/deionized water and bring the volume to 100.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add oatmeal to distilled/deionized water
and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to
boiling. Steam for 20 min. Filter through cheesecloth. Add agar. Add
sufficient distilled/deionized water to bring volume to 999.0mL. Gen-
tly heat and bring to boiling. Mix thoroughly. Distribute into tubes or
flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add 1.0mL of sterile trace salts solution. Mix thor-
oughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Streptomyces species according to the
International Streptomyces Project.
(International Streptomyces Project Medium 3)
(Oatmeal Agar)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Oatmeal.......................................................................................20.0gAgar............................................................................................18.0g
Trace salts solution ....................................................................1.0mL
Trace Salts Solution:
Composition per 100.0mL:
FeSO 4 ·7H 2 O..................................................................................0.1g
MnCl 2 ·4H 2 O .................................................................................0.1g
ZnSO 4 ·7H 2 O.................................................................................0.1g
Preparation of Trace Salts Solution: Add components to dis-
tilled/deionized water and bring the volume to 100.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add oatmeal to distilled/deionized water
and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to
boiling. Steam for 20 min. Filter through cheesecloth. Add agar. Add
sufficient distilled/deionized water to bring volume to 999.0mL. Gen-
tly heat and bring to boiling. Mix thoroughly. Distribute into tubes or
flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add 1.0mL of sterile trace salts solution. Mix thor-
oughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Streptomyces species according to the
International Streptomyces Project.
