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Isosphaera Agar
[所属分类:培养基配方] [发布时间:2021-4-21] [发布人:李佳颐] [阅读次数:] [返回]
Isosphaera Agar
Solution B..............................................................................100.0mL
Solution C..............................................................................100.0mL
Vitamin solution.........................................................................0.5mL
pH 7.6 ± 0.2 at 25°C
Solution A:
Composition per 800.0mL:
Agar, noble..................................................................................15.0g
NaCl............................................................................................0.25g
(NH 4 ) 2 SO 4 ................................................................................0.125g
KCl............................................................................................0.125g
MgSO 4 ·7H 2 O................................................................................0.1g
CaCl 2 ·2H 2 O .............................................................................80.0mg
KH 2 PO 4 ....................................................................................75.0mg
FeCl 3 .........................................................................................73.0μg
Trace elements solution SL-7....................................................2.5mL
Trace Elements Solution SL-7:
Composition per liter:
FeCl 2 ·4H 2 O...................................................................................1.5g
CoCl 2 ·6H 2 O...........................................................................190.0mg
MnCl 2 ·4H 2 O ..........................................................................100.0mg
ZnCl 2 ........................................................................................70.0mg
H 3 BO 3 ......................................................................................62.0mg
Na 2 MoO 4 ·2H 2 O.......................................................................36.0mg
NiCl 2 ·6H 2 O..............................................................................24.0mg
CuCl 2 ·2H 2 O.............................................................................17.0mg
HCl (25% solution)..................................................................10.0mL
Preparation of Trace Elements Solution SL-7: Add FeCl 2 ·4H 2 O
to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized
water and bring volume to 1.0L. Add remaining components. Mix thor-
oughly.
Preparation of Solution A: Add agar to 400.0mL of distilled/de-
ionized water. In another flask, add remaining components to 400.0mL
of distilled/deionized water. Mix thoroughly. Adjust pH to 7.6 with
NaOH. Remove any precipitate that forms by filtering through What-
man #1 filter paper. Autoclave both solutions separately for 15 min at
15 psi pressure–121°C. Aseptically combine the two sterile solutions.
Cool to 50°–55°C.
Solution B:
Composition per 100.0mL:
NaHCO 3 ......................................................................................0.42g
Preparation of Solution B: Add NaHCO 3 to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Glucose .......................................................................................0.25g
Casamino acids...........................................................................0.25g
Preparation of Solution C: Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Warm to 50°–55°C.
Vitamin Solution:
Composition per 100.0mL:
Nicotinic acid.........................................................................200.0mg
Thiamine HCl ........................................................................200.0mg
p-Aminobenzoic acid...............................................................20.0mg
Biotin .........................................................................................2.0mg
Vitamin B 12 ...............................................................................25.0μg
Preparation of Vitamin Solution: Add components to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter steril-
ize. Warm to 50°–55°C.
Preparation of Medium: Aseptically combine 800.0mL of sterile
solution A with 100.0mL of sterile solution B, 100.0mL of sterile so-
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per 1000.5mL:
Solution A..............................................................................800.0mLSolution B..............................................................................100.0mL
Solution C..............................................................................100.0mL
Vitamin solution.........................................................................0.5mL
pH 7.6 ± 0.2 at 25°C
Solution A:
Composition per 800.0mL:
Agar, noble..................................................................................15.0g
NaCl............................................................................................0.25g
(NH 4 ) 2 SO 4 ................................................................................0.125g
KCl............................................................................................0.125g
MgSO 4 ·7H 2 O................................................................................0.1g
CaCl 2 ·2H 2 O .............................................................................80.0mg
KH 2 PO 4 ....................................................................................75.0mg
FeCl 3 .........................................................................................73.0μg
Trace elements solution SL-7....................................................2.5mL
Trace Elements Solution SL-7:
Composition per liter:
FeCl 2 ·4H 2 O...................................................................................1.5g
CoCl 2 ·6H 2 O...........................................................................190.0mg
MnCl 2 ·4H 2 O ..........................................................................100.0mg
ZnCl 2 ........................................................................................70.0mg
H 3 BO 3 ......................................................................................62.0mg
Na 2 MoO 4 ·2H 2 O.......................................................................36.0mg
NiCl 2 ·6H 2 O..............................................................................24.0mg
CuCl 2 ·2H 2 O.............................................................................17.0mg
HCl (25% solution)..................................................................10.0mL
Preparation of Trace Elements Solution SL-7: Add FeCl 2 ·4H 2 O
to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized
water and bring volume to 1.0L. Add remaining components. Mix thor-
oughly.
Preparation of Solution A: Add agar to 400.0mL of distilled/de-
ionized water. In another flask, add remaining components to 400.0mL
of distilled/deionized water. Mix thoroughly. Adjust pH to 7.6 with
NaOH. Remove any precipitate that forms by filtering through What-
man #1 filter paper. Autoclave both solutions separately for 15 min at
15 psi pressure–121°C. Aseptically combine the two sterile solutions.
Cool to 50°–55°C.
Solution B:
Composition per 100.0mL:
NaHCO 3 ......................................................................................0.42g
Preparation of Solution B: Add NaHCO 3 to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Warm to 50°–55°C.
Solution C:
Composition per 100.5mL:Glucose .......................................................................................0.25g
Casamino acids...........................................................................0.25g
Preparation of Solution C: Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Warm to 50°–55°C.
Vitamin Solution:
Composition per 100.0mL:
Nicotinic acid.........................................................................200.0mg
Thiamine HCl ........................................................................200.0mg
p-Aminobenzoic acid...............................................................20.0mg
Biotin .........................................................................................2.0mg
Vitamin B 12 ...............................................................................25.0μg
Preparation of Vitamin Solution: Add components to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter steril-
ize. Warm to 50°–55°C.
Preparation of Medium: Aseptically combine 800.0mL of sterile
solution A with 100.0mL of sterile solution B, 100.0mL of sterile so-
lution C, and 0.5mL of sterile vitamin solution. Mix thoroughly. Pour
into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Isosphaera pallida.