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Isonema Medium
[所属分类:培养基配方] [发布时间:2021-4-21] [发布人:李佳颐] [阅读次数:] [返回]
Isonema Medium
Seawater.................................................................................990.0mL
Horse serum, heat inactivated..................................................10.0mL
Preparation of Medium: Add pancreatic digest of casein to seawa-
ter and bring volume to 990.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 10.0mL
of heat-inactivated horse serum. Mix thoroughly. Aseptically distribute
into sterile tubes or flasks.
Use: For the cultivation of Isonema papillatum.
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Pancreatic digest of casein............................................................1.0gSeawater.................................................................................990.0mL
Horse serum, heat inactivated..................................................10.0mL
Preparation of Medium: Add pancreatic digest of casein to seawa-
ter and bring volume to 990.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 10.0mL
of heat-inactivated horse serum. Mix thoroughly. Aseptically distribute
into sterile tubes or flasks.
Use: For the cultivation of Isonema papillatum.
