ISM Broth

[所属分类:培养基配方] [发布时间:2021-4-21] [发布人:李佳颐] [阅读次数:] [返回]

ISM Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

MgSO 4 ·7H 2 O..............................................................................49.2g
NaCl............................................................................................43.5g
Yeast extract..................................................................................4.0g
Peptone .........................................................................................2.0g
CaCl 2 ·2H 2 O ..................................................................................1.5g
Maltose solution.....................................................................100.0mL
Maltose Solution:
Composition per 100.0mL:
Maltose .........................................................................................5.0g
Preparation of Maltose Solution:  Add maltose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except maltose solu-
tion, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile malt-
ose solution. Mix thoroughly. Aseptically distribute into sterile tubes or
flasks.
Use: For the cultivation of Spirochaeta halophila.