Islams Medium Base for Group B Streptococci

[所属分类:培养基配方] [发布时间:2021-4-21] [发布人:李佳颐] [阅读次数:] [返回]

Islams Medium Base for Group B Streptococci

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Proteose peptone.........................................................................23.0g
Agar ............................................................................................10.0g
Na 2 HPO 4 ...................................................................................5.749g
Starch, soluble...............................................................................5.0g
KH 2 PO 4 .....................................................................................1.482g
Horse serum, sterile inactivated...............................................50.0mL
pH 7.4 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Preparation of Medium: Add components, except horse serum, to
distilled/deionized water and bring volume to 950.0mL. Mix thorough-
ly. Autoclave for 15 min at 10 psi pressure–115°C. Cool to 50°C. Asep-
tically add horse serum. Mix thoroughly. Pour into Petri dishes or
aseptically distribute into sterile tubes.
Use: For the identification and cultivation of Group B streptococci
from clinical specimens.