Iron Milk Medium

[所属分类:培养基配方] [发布时间:2021-4-21] [发布人:李佳颐] [阅读次数:] [返回]

Iron Milk Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Iron filings.....................................................................................1.0g
Whole milk ...................................................................................1.0L
pH 6.8 ± 0.2 at 25°C
Preparation of Medium: Add iron filings, which may be small
balls of steel wool, to whole milk and bring volume to 1.0L. Mix thor-

oughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi

pressure–121°C.

Use: For the cultivation of lactic acid bacteria. For the cultivation and
differentiation of Clostridium species. The medium turns black if H 2 S
is produced. The medium turns red if acid is produced from milk car-
bohydrate fermentation. Acid and gas production is characteristic of
Clostridium perfringens and Clostridium butyricum.