Iron Bacteria Isolation Medium

[所属分类:培养基配方] [发布时间:2021-4-21] [发布人:李佳颐] [阅读次数:] [返回]

Iron Bacteria Isolation Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................10.0g
(NH 4 ) 2 SO 4 .....................................................................................0.5g
Glucose .......................................................................................0.15g
CaCO 3 ...........................................................................................0.1g
K 2 HPO 4 .......................................................................................0.05g
MgSO 4 ·7H 2 O..............................................................................0.05g
KCl..............................................................................................0.05g
Ca(NO 3 ) 2 .....................................................................................0.01g
Vitamin solution.......................................................................10.0mL
Vitamin Solution:
Composition per 10.0mL:
Thiamine....................................................................................0.4mg
Cyanocobalamin ......................................................................0.01mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except vitamin solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL of
vitamin solution. Mix thoroughly. Pour into sterile Petri dishes or dis-
tribute into sterile tubes.
Use: For the isolation of iron bacteria.