Irgasan ® Ticarcillin Chlorate Broth (ITC Broth)

[所属分类:培养基配方] [发布时间:2021-4-21] [发布人:李佳颐] [阅读次数:] [返回]

Irgasan ® Ticarcillin Chlorate Broth
(ITC Broth)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

MgCl 2 ·6H 2 0................................................................................60.0g
Pancreatic digest of casein..........................................................10.0g
NaCl..............................................................................................5.0g
KClO 4 ...........................................................................................1.0g
Yeast extract..................................................................................1.0g
Malachite Green (0.2% solution)...............................................5.0mL
Irgasan solution..........................................................................1.0mL
Ticarcillin solution.....................................................................1.0mL
pH 7.6 ± 0.2 at 25°C
Irgasan Solution:
Composition per 10.0mL:
Irgasan (triclosan)......................................................................1.0mg
Preparation of Irgasan Solution: Add Irgasan to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-
ize.
Ticarcillin Solution:
Composition per 10.0mL:
Ticarcillin..................................................................................1.0 mg
Preparation of Ticarcillin Solution: Add ticarcillin to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except Irgasan solution
and ticarcillin solution, to distilled/deionized water and bring volume
to 998.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C. Adjust to pH 7.6. Aseptically add 1.0mL of
Irgasan solution and 1.0mL of ticarcillin solution. Mix thoroughly.
Aseptically distribute into sterile tubes or flasks.
Use: For the selective isolation and cultivation of Yersinia species.