Ionic Medium with Pipecolate

[所属分类:培养基配方] [发布时间:2021-4-21] [发布人:李佳颐] [阅读次数:] [返回]

Ionic Medium with Pipecolate

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................30.0g
Ionic medium.........................................................................950.0mL
Pipecolic acid·HCl solution .....................................................50.0mL
Ionic Medium:
Composition per liter:
K 2 HPO 4 .........................................................................................4.1g
Na 2 HPO 4 .....................................................................................3.34g
KH 2 PO 4 .......................................................................................2.26g
NaH 2 PO 4 .....................................................................................2.24g

Salt solution .............................................................................10.0mL

Preparation of Ionic Medium: Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly.
Salt Solution:
Composition per liter:
MgSO 4 ·7H 2 O..............................................................................14.8g
FeSO 4 ·7H 2 O................................................................................0.55g
MnSO 4 ......................................................................................0.045g
H 2 SO 4 , concentrated..................................................................0.2mL
Preparation of Salt Solution: Add components to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly.
Pipecolic Acid·HCl Solution:
Composition per 100.0mL:
Pipecolic acid·HCl......................................................................4.14g
Preparation of Pipecolic Acid·HCl Solution: Add pipecolic
acid·HCl to distilled/deionized water and bring volume to 100.0mL.
Mix thoroughly. Adjust pH to 7.0.
Preparation of Medium: Add agar to 950.0mL of ionic medium.
Mix thoroughly. Gently heat and bring to boiling. Add 50.0mL of pipe-
colic acid·HCl. Mix thoroughly. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes
or leave in tubes.
Use:  For the cultivation and maintenance of Pseudomonas putida.