Infection Medium (IM)

[所属分类:培养基配方] [发布时间:2021-4-20] [发布人:李佳颐] [阅读次数:] [返回]

Infection Medium
(IM)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 100.0mL:

Pancreatic digest of gelatin.........................................................0.05g
Bile salts No. 3............................................................................0.05g
Brain heart, solids from infusion................................................0.02g
Peptic digest of animal tissue .....................................................0.02g
NaCl..........................................................................................0.017g
Glucose.......................................................................................0.01g
Na 2 HPO 4 ....................................................................................8.0mg
Earle’s balanced salts solution.................................................80.0mL
Fetal bovine serum, heat inactivated (2 hr at 55°C)................20.0mL
pH 7.4 ± 0.2 at 25°C
Earle’s Balanced Salts Solution:
Composition per liter:
NaCl..............................................................................................6.8g
NaHCO 3 ........................................................................................2.2g
Glucose .........................................................................................1.0g
KCl................................................................................................0.4g
CaCl 2 ·2H 2 O...............................................................................0.265g
MgSO 4 ·7H 2 O................................................................................0.2g
NaH 2 PO 4 ·H 2 O.............................................................................0.14g
Preparation of Earle’s Balanced Salts Solution:  Add compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Filter sterilize.
Preparation of Medium:  Combine components. Mix thoroughly.
Filter sterilize. Store at 4°–10°C.
Use: For the screening of Escherichia coli for pathogenicity using the
HeLa cell test for invasiveness.