Indole Nitrate Medium (Trypticase™ Nitrate Broth)

[所属分类:培养基配方] [发布时间:2021-4-20] [发布人:李佳颐] [阅读次数:] [返回]

Indole Nitrate Medium
(Trypticase™ Nitrate Broth)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein..........................................................20.0g
Na 2 HPO 4 .......................................................................................2.0g
Agar..............................................................................................1.0g
Glucose.........................................................................................1.0g
KNO 3 ............................................................................................1.0g
pH 7.2 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling with frequent agitation. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C.
Use: For the identification of microorganisms by means of the nitrate
reduction and indole tests