Hyphomicrobium Medium

[所属分类:培养基配方] [发布时间:2021-4-19] [发布人:李佳颐] [阅读次数:] [返回]

Hyphomicrobium Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Noble agar...................................................................................18.0g
Na 2 HPO 4 .....................................................................................2.15g
KH 2 PO 4 .......................................................................................1.36g
(NH 4 ) 2 SO 4 .....................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.2g
Trace elements solution .............................................................5.0mL
Methylamine·HCl solution.......................................................20.0mL

pH 7.1 ± 0.1 at 25°C

Trace Elements Solution:

Composition per 100.0mL:
CuCl 2 ..........................................................................................0.15g
FeSO 4 ·7H 2 O..................................................................................0.1g
Na 2 MoO 4 ·2H 2 O..........................................................................0.05g
MnSO 4 ·H 2 O..............................................................................0.035g
Preparation of Trace Elements Solution : Add components to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly.
Methylamine·HCl Solution:
Composition per 20.0mL:
Methylamine·HCl .......................................................................3.38g
Preparation of Methylamine·HCl Solution: Add methyl-
amine·HCl to distilled/deionized water and bring volume to 20.0mL.
Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except methylamine·HCl
solution, to distilled/deionized water and bring volume to 980.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15
psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile
methylamine·HCl solution. Mix thoroughly. Adjust pH to 7.1, if necessary.
Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Hyphomicrobium aestu-
arii, Hyphomicrobium facilis, Hyphomicrobium hollandicum, Hypho-
microbium vulgare, and Hyphomicrobium zavarzinii.