Hyphomicrobium Medium

[所属分类:培养基配方] [发布时间:2021-4-19] [发布人:李佳颐] [阅读次数:] [返回]

Hyphomicrobium Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Na 2 HPO 4 .....................................................................................2.13g
KH 2 PO 4 .......................................................................................1.36g
MgSO 4 ·7H 2 O................................................................................0.2g
CaCl 2 ·2H 2 O..............................................................................9.95mg
FeSO 4 ·7H 2 O...............................................................................5.0mg
MnSO 4 ·4H 2 O.............................................................................2.5mg
Na 2 MoO 4 ·2H 2 O .........................................................................2.5mg
Urea solution............................................................................30.0mL
Methanol....................................................................................4.0mL
Urea Solution:
Composition per 100.0mL:
Urea.............................................................................................20.0g
Preparation of Urea Solution: Add urea to distilled/deionized wa-
ter and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Filter sterilize methanol. Add compo-
nents, except urea solution and methanol, to distilled/deionized water
and bring volume to 966.0mL. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C. Aseptically add sterile urea solution and sterile methanol.
Mix thoroughly. Aseptically distribute into sterile tubes or bottles.
Use: For the cultivation of Hyphomicrobium species.