Hydroxybenzoate Medium

[所属分类:培养基配方] [发布时间:2021-4-19] [发布人:李佳颐] [阅读次数:] [返回]

Hydroxybenzoate Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Noble agar...................................................................................20.0g
(NH 4 ) 2 HPO 4 ..................................................................................3.0g
K 2 HPO 4 .........................................................................................1.2g
NaCl..............................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.2g
FeSO 4 ·7H 2 O..................................................................................0.1g
p-Hydroxybenzoic acid solution..............................................50.0mL
pH 7.0 ± 0.2 at 25°C
p-Hydroxybenzoic Acid Solution:
Composition per 50.0mL:
p-Hydroxybenzoic acid.................................................................3.0g
Preparation of p-Hydroxybenzoic Acid Solution: Add p-hy-
droxybenzoic acid to distilled/deionized water and bring volume to
50.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except p-hydroxyben-
zoic acid solution, to distilled/deionized water and bring volume to
950.0mL. Mix thoroughly. Gently heat and bring to boiling. Adjust pH
to 7.0 with 5N NaOH. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 45°–50°C. Aseptically add sterile p-hydroxybenzoic acid solu-
tion. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use:  For the cultivation and maintenance of Pseudomonas putida.