Hydroxybenzoate Broth

[所属分类:培养基配方] [发布时间:2021-4-19] [发布人:李佳颐] [阅读次数:] [返回]

Hydroxybenzoate Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1001.0mL:

Solution A..............................................................................990.0mL
Solution B................................................................................10.0mL
Solution C..................................................................................1.0mL
pH 7.0 ± 0.2 at 25°C
Solution A:
Composition per 990.0mL:
4-Hydroxybenzoic acid.................................................................3.0g
(NH 4 ) 2 SO 4 ....................................................................................3.0g
NaCl..............................................................................................2.5g
K 2 HPO 4 .........................................................................................1.6g
Yeast extract..................................................................................0.5g
Preparation of Solution A: Add components to distilled/deionized
water and bring volume to 990.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C.
Solution B:
Composition per 10.0mL:
MgSO 4 ·7H 2 O..............................................................................0.27g
Preparation of Solution B: Add MgSO 4 ·7H 2 O to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for
15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Solution C:
Composition per 1.0mL:
Fe(NH 4 ) 2 (SO 4 ) 2 ·6H 2 O ................................................................0.05g
Preparation of Solution C: Add component to distilled/deionized
water and bring volume to 1.0mL. Mix thoroughly. Filter sterilize. Pre-
pare solution immediately before adding to solutions A and B.
Preparation of Medium: Aseptically combine cooled sterile solution
A and cooled sterile solution B. Immediately add 1.0mL of freshly pre-
pared sterile solution C. Adjust pH to 7.0 with 6N NaOH. Mix thoroughly.
Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Comamonas testosteroni.