Hydrogenobacter halophilus Medium (DSMZ Medium 744)

[所属分类:培养基配方] [发布时间:2021-4-19] [发布人:李佳颐] [阅读次数:] [返回]

Hydrogenobacter halophilus Medium
(DSMZ Medium 744)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................29.3g
K 2 HPO 4 .........................................................................................2.5g
(NH 4 ) 2 SO 4 .....................................................................................2.0g
KH 2 PO 4 .........................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.2g
CaCl 2 ........................................................................................10.0mg
FeSO 4 ·7H 2 O.............................................................................10.0mg
NiSO 4 ·7H 2 O...............................................................................0.6mg
Trace elements solution .............................................................0.5mL
pH 6.9 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
ZnSO 4 ·7H 2 O............................................................................28.0mg
MoO 3 ..........................................................................................4.0mg
H 3 BO 3 ........................................................................................4.0mg
MnSO 4 ·5H 2 O.............................................................................4.0mg
CoCl 2 ·6H 2 O ...............................................................................4.0mg
CuSO 4 ·5H 2 O..............................................................................2.0mg
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Sparge with 80% H 2 + 20% CO 2 .
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min
at 15 psi pressure–121°C. Adjust pH to 6.9. Aseptically distribute into
sterile tubes or flasks.
Use: For the cultivation of Hydrogenovibrio marinus.