Hydrogenobacter acidophilus Medium (DSMZ Medium 743)

[所属分类:培养基配方] [发布时间:2021-4-19] [发布人:李佳颐] [阅读次数:] [返回]

Hydrogenobacter acidophilus Medium
(DSMZ Medium 743)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Sulfur............................................................................................5.0g
(NH 4 ) 2 SO 4 ....................................................................................1.0g
K 2 HPO 4 .........................................................................................1.0g
NaCl..............................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.3g
FeSO 4 ·7H 2 O...............................................................................1.0mg
CaCl 2 .........................................................................................1.0mg
NiSO 4 ·6H 2 O.............................................................................0.06mg
Trace elements solution.............................................................0.5mL
pH 3.0 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
ZnSO 4 ·7H 2 O............................................................................28.0mg
MoO 3 ..........................................................................................4.0mg
H 3 BO 3 ........................................................................................4.0mg
MnSO 4 ·5H 2 O.............................................................................4.0mg
CoCl 2 ·6H 2 O ...............................................................................4.0mg
CuSO 4 ·5H 2 O..............................................................................2.0mg
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Sparge with 80% H 2 + 20% CO 2 .
Preparation of Medium: Autoclave sulfur for 15 min at 9 psi pres-
sure–113°C. Add components, except sulfur, to distilled/deionized wa-
ter and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at
15 psi pressure–121°C. Add 5.0g sterile sulfur. Mix thoroughly by
swirling. Adjust pH to 3.0 with HCl. Aseptically distribute into sterile
tubes or flasks.
Use: For the cultivation of Hydrogenobaculum acidophilum=Hydrog-
enobacter acidophilus