Hydrogen-Oxidizing Bacteria Medium

[所属分类:培养基配方] [发布时间:2021-4-16] [发布人:李佳颐] [阅读次数:] [返回]

Hydrogen-Oxidizing Bacteria Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1020.0mL:

Solution I ......................................................................................1.0L
Solution II................................................................................10.0mL
Solution III...............................................................................10.0mL
Solution I:
Composition per liter:
Na 2 HPO 4 ·12H 2 O...........................................................................9.0g
KH 2 PO 4 .........................................................................................1.5g
NH 4 Cl ...........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.2g
Trace elements solution.............................................................1.0mL
Preparation of Solution I: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat until dis-

solved. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Trace Elements Solution:
Composition per liter:
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O ..................................................................................0.2g
ZnSO 4 ·7H 2 O.................................................................................0.1g
MnCl 2 ·4H 2 O................................................................................0.03g
NaMoO 4 ·2H 2 O............................................................................0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 ·2H 2 O ................................................................................0.01g
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Solution II:
Composition per 100.0mL:
CaCl 2 ·2H 2 O...................................................................................0.1g
Ferric ammonium citrate.............................................................0.05g
Preparation of Solution II: Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 25°C.
Solution III:
Composition per 100.0mL:
NaHCO 3 ........................................................................................5.0g
Preparation of Solution III: Add NaHCO 3 to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium:  Aseptically combine 1.0L of cooled, ster-
ile solution I, 10.0mL of cooled, sterile solution II, and 10.0mL of ster-
ile solution III. Mix thoroughly. Aseptically distribute into sterile tubes
or flasks.
Use: For the cultivation of hydrogen-oxidizing bacteria.