HiCrome™ Rapid Coliform Broth (Coliform Rapid HiCrome™ Broth) (Rapid Coliform HiCrome™ Broth)

[所属分类:培养基配方] [发布时间:2021-4-9] [发布人: ] [阅读次数:] [返回]

HiCrome™ Rapid Coliform Broth
(Coliform Rapid HiCrome™ Broth)

(Rapid Coliform HiCrome™ Broth)

培养基配方

Composition per liter:
Peptone, special ............................................................................5.0g
NaCl..............................................................................................5.0g
Na 2 HPO 4 .......................................................................................2.7g
KH 2 PO 4 .........................................................................................2.0g
Sorbitol .........................................................................................1.0g
Sodium lauryl sulfate....................................................................0.1g
IPTG .............................................................................................0.1g
Chromogenic substrate ...............................................................0.08g
Fluorogenic substrate..................................................................0.05g
pH 6.8 ± 0.3 at 25°C
Source: This medium is available as a premixed powder from HiMedia.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes.
Use: For the detection and confirmation of Escherichia coli and coliforms on the basis of enzyme substrate reaction from water samples, using a combination of chromogenic and fluorogenic substrate.